7STQ
Crystal structure of arabidopsis thaliana acetohydroxyacid synthase W574L mutant in complex with chlorimuron-ethyl
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-07 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 178.129, 178.129, 184.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.130 - 3.300 |
| R-factor | 0.1802 |
| Rwork | 0.178 |
| R-free | 0.20530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k2o |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.130 | 3.530 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rpim | 0.044 | 0.197 |
| Number of reflections | 26470 | 4647 |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 99.6 | 98.7 |
| Redundancy | 10.7 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.8 | 293 | CHES buffer, potassium sodium tartrate, sodium sulfate |
