7STE
Rad24-RFC ADP state
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.73 |
Number of Particle | 4432412 |
Software | cryoSPARC,SerialEM,CTFFIND,Coot,PHENIX |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |