7SSU
Structure of EmrE-D3 mutant in complex with monobody L10 and methyltriphenylphosphonium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2021-09-27 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9183 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 141.171, 50.870, 110.799 |
Unit cell angles | 90.00, 92.69, 90.00 |
Refinement procedure
Resolution | 58.240 - 3.220 |
R-factor | 0.2944 |
Rwork | 0.292 |
R-free | 0.33280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6wk8 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2-3874-000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.500 | 3.420 |
High resolution limit [Å] | 3.220 | 3.220 |
Rmerge | 0.152 | 0.656 |
Rmeas | 0.707 | |
Number of reflections | 8969 | 448 |
<I/σ(I)> | 10.4 | |
Completeness [%] | 88.6 | 13.22 |
Redundancy | 6.6 | |
CC(1/2) | 0.967 | 0.861 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.1 M lithium nitrate, 0.1 M ADA, pH 6.5, 33% PEG600 |