7SSF

Light harvesting phycobiliprotein HaPE560 from the cryptophyte Hemiselmis andersenii CCMP644

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-23
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	I 1 2 1
Unit cell lengths	84.320, 67.970, 184.494
Unit cell angles	90.00, 99.33, 90.00

Refinement procedure

Resolution	28.940 - 1.450
R-factor	0.156
Rwork	0.154
R-free	0.19210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lmx
RMSD bond length	0.013
RMSD bond angle	1.415
Data reduction software	iMOSFLM
Data scaling software	pointless (1.11.17)
Phasing software	PHENIX (1.14)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.940	1.470
High resolution limit [Å]	1.450	1.450
Rmerge	0.095	1.424
Number of reflections	181639	8893
<I/σ(I)>	12.5	1.4
Completeness [%]	99.8	99.3
Redundancy	7.4	7.2
CC(1/2)	0.999	0.546

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	300	TRIS HCl 0.162 M + PEGMME 5000 21.4 % (v/v)