7SR3
Single chain trimer HLA-A*02:01 (H98L, Y108C) with HPV.16 E7 peptide YMLDLQPETTDL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2020-10-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 118.093, 118.093, 262.205 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.990 - 2.490 |
R-factor | 0.226 |
Rwork | 0.225 |
R-free | 0.24770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7sqp |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.580 |
High resolution limit [Å] | 2.490 | 2.490 |
Number of reflections | 65593 | 6332 |
<I/σ(I)> | 19.7 | |
Completeness [%] | 99.9 | |
Redundancy | 8.1 | |
CC(1/2) | 0.996 | 0.847 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 200 mM K2SO4, 17% PEG 3350 |