7SPZ
Nucleotide-free Get3 in two open forms
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 54.138, 102.605, 138.801 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.110 - 3.000 |
R-factor | 0.2958 |
Rwork | 0.293 |
R-free | 0.34830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ibg |
RMSD bond length | 0.002 |
RMSD bond angle | 1.152 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.820 |
High resolution limit [Å] | 2.770 | 2.770 |
Number of reflections | 17087 | 997 |
<I/σ(I)> | 17.9 | |
Completeness [%] | 84.2 | |
Redundancy | 11.5 | |
CC(1/2) | 0.994 | 0.511 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 277.15 | 0.1 M MES, pH 5.3, 0.1 M magnesium chloride, 21% PEG3350 |