7SPD
Crystal Structure of The Tetramerization Domain (29-147) From Human Voltage-gated Potassium Channel Kv2.1 in C 2 2 21 Space Group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2020-11-06 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 107.290, 114.120, 112.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.670 - 2.700 |
R-factor | 0.2136 |
Rwork | 0.212 |
R-free | 0.24820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kvt |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.430 | 48.430 | 2.830 |
High resolution limit [Å] | 2.700 | 8.960 | 2.700 |
Rmerge | 0.113 | 0.025 | 1.292 |
Rmeas | 0.122 | 0.028 | 1.400 |
Rpim | 0.045 | 0.010 | 0.537 |
Total number of observations | 3923 | 16901 | |
Number of reflections | 36640 | 592 | 2534 |
<I/σ(I)> | 17.2 | 59.5 | 1.5 |
Completeness [%] | 100.0 | 99.4 | 100 |
Redundancy | 7.1 | 6.6 | 6.7 |
CC(1/2) | 0.998 | 0.999 | 0.638 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.2 M magnesium chloride, 15% PEG400, 0.1 M HEPES, pH 7.5 |