7SP3

E. coli RppH bound to Ap4A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU R-AXIS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-09-23
Detector	RIGAKU
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	79.390, 36.384, 57.801
Unit cell angles	90.00, 102.10, 90.00

Refinement procedure

Resolution	18.840 - 1.600
R-factor	0.1846
Rwork	0.183
R-free	0.21950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4s2w
RMSD bond length	0.007
RMSD bond angle	0.957
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.400	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.043	0.587
Rmeas	0.048	0.665
Rpim	0.021	0.305
Number of reflections	20993	1890
<I/σ(I)>	22.49	2.6
Completeness [%]	97.5	89.32
Redundancy	5.2	4.4
CC(1/2)	1.000	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.4 M (NH4)2SO4, 10% (v/v) PEG3350, and 10% glycerol