7SOZ
Replication Initiator Protein REPE54 and cognate DNA sequence with terminal three prime phosphates chemically crosslinked (5 mg/mL EDC, 12 hours).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2021-04-16 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 111.993, 79.181, 74.903 |
Unit cell angles | 90.00, 123.02, 90.00 |
Refinement procedure
Resolution | 37.050 - 3.140 |
R-factor | 0.1972 |
Rwork | 0.193 |
R-free | 0.26320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7rva |
RMSD bond length | 0.012 |
RMSD bond angle | 1.480 |
Data reduction software | XDS (Jan 31, 2020) |
Data scaling software | XSCALE (Jan 31, 2020) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.050 | 3.360 |
High resolution limit [Å] | 3.140 | 3.140 |
Rmerge | 0.085 | 0.408 |
Rmeas | 0.100 | 0.480 |
Rpim | 0.052 | 0.251 |
Number of reflections | 9433 | 1662 |
<I/σ(I)> | 6.6 | 1.8 |
Completeness [%] | 97.3 | 96.2 |
Redundancy | 3.6 | 3.6 |
CC(1/2) | 0.997 | 0.965 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 90 mM MgCl2, 3% PEG 400, and 220 mM Tris HCl. |