7SOW

LaM domain of human LARP1 in complex with UUUUUU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-09
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.96860
Spacegroup name	P 21 21 21
Unit cell lengths	36.804, 46.885, 57.904
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.440 - 1.300
R-factor	0.1829
Rwork	0.182
R-free	0.19210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7soo
RMSD bond length	0.014
RMSD bond angle	1.486
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.320
High resolution limit [Å]	1.300	1.300
Number of reflections	24922	2179
<I/σ(I)>	24.7
Completeness [%]	98.8
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M ammonium acetate, 0.1 M Bis-Tris pH 5.5, 25% PEG3350