7SOP

LaM domain of human LARP1 in complex with AAAUAA RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.52154
Spacegroup name	P 21 21 21
Unit cell lengths	36.521, 46.259, 57.374
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.010 - 1.550
R-factor	0.197
Rwork	0.194
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7soo
RMSD bond length	0.013
RMSD bond angle	1.343
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.550	1.550
Number of reflections	14361	1208
<I/σ(I)>	29
Completeness [%]	98.6
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES pH 7.5, 25% (w/v) PEG 3350