7SOJ

Structure of V750A Soybean Lipoxygenase at 277K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	277
Detector technology	PIXEL
Collection date	2017-07-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.88557
Spacegroup name	P 1 21 1
Unit cell lengths	91.537, 92.693, 100.270
Unit cell angles	90.00, 93.75, 90.00

Refinement procedure

Resolution	46.350 - 1.850
R-factor	0.17
Rwork	0.169
R-free	0.19840
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5t5v
RMSD bond length	0.005
RMSD bond angle	0.820
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.350	1.880
High resolution limit [Å]	1.850	1.850
Rmerge	0.211	2.460
Rpim	0.092	1.570
Number of reflections	141110	6896
<I/σ(I)>	5.7
Completeness [%]	99.1
Redundancy	6.8
CC(1/2)	0.992	0.469

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		273	PEG 3350 acetate