7SNK
Structure of Bacple_01702, a GH29 family glycoside hydrolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-15 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 54.450, 77.430, 133.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.540 - 2.000 |
R-factor | 0.1725 |
Rwork | 0.170 |
R-free | 0.21380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ni3 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.841 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19rc4_4035) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.540 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 38905 | 5643 |
<I/σ(I)> | 22.2 | 5.11 |
Completeness [%] | 99.6 | |
Redundancy | 23.8 | |
CC(1/2) | 0.990 | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% Peg8K, 0.1M Potassium dihydrogen phosphate |