7SMC

p107 pocket domain complexed with ARID4A peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 1
Unit cell lengths	62.863, 62.923, 71.274
Unit cell angles	69.89, 76.00, 74.36

Refinement procedure

Resolution	47.110 - 2.700
R-factor	0.2407
Rwork	0.238
R-free	0.29820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yos
RMSD bond length	0.010
RMSD bond angle	1.854
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.110	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.160	0.530
Number of reflections	26604	3491
<I/σ(I)>	5.3	2
Completeness [%]	99.3
Redundancy	3.6
CC(1/2)	0.970	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100 mM MES, pH 6.5, 4% PEG400, 1.6 M ammonium sulfate