7SLR
HIV Reverse Transcriptase with compound Pyr01
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-05 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 117.427, 154.243, 155.561 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 93.430 - 2.179 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.23210 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.7 (18-SEP-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.430 | 2.315 |
| High resolution limit [Å] | 2.179 | 2.179 |
| Number of reflections | 58052 | 2906 |
| <I/σ(I)> | 19 | |
| Completeness [%] | 78.7 | |
| Redundancy | 6.3 | |
| CC(1/2) | 1.000 | 0.589 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 950 mM sodium malonate pH 7.0, 100 mM HEPES/NaOH pH 6.8 |
