7SL5
Crystal Structure of VP12E7 Fab in complex with SARS-CoV-2 S fusion peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2021-04-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9774 |
Spacegroup name | P 1 |
Unit cell lengths | 71.148, 81.359, 95.650 |
Unit cell angles | 90.34, 95.96, 100.65 |
Refinement procedure
Resolution | 48.200 - 2.500 |
R-factor | 0.212 |
Rwork | 0.210 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7r6w |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.200 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.100 | 0.991 |
Number of reflections | 70930 | 70929 |
<I/σ(I)> | 6.9 | |
Completeness [%] | 97.8 | |
Redundancy | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 295 | 0.2 M Calcium Acetate Hydrate, 0.1 M MES NaOH, pH 6.0, 25% PEG 8000 |