7SKU
Nipah virus matrix protein in complex with PI(4,5)P2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03317 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.114, 96.471, 119.972 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.180 - 2.117 |
| R-factor | 0.2327 |
| Rwork | 0.231 |
| R-free | 0.26420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7skt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS (Feb 5, 2021) |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 75.180 | 75.180 | 2.154 |
| High resolution limit [Å] | 2.117 | 5.746 | 2.117 |
| Rmerge | 0.201 | 0.047 | 3.308 |
| Rmeas | 0.209 | 0.049 | 3.460 |
| Rpim | 0.058 | 0.014 | 0.996 |
| Total number of observations | 579838 | 29619 | 26354 |
| Number of reflections | 45637 | 2544 | 2288 |
| <I/σ(I)> | 9.95 | 35.79 | 0.72 |
| Completeness [%] | 96.9 | 99.9 | 100 |
| Redundancy | 12.71 | 11.64 | 11.52 |
| CC(1/2) | 0.998 | 0.999 | 0.380 |
| Anomalous redundancy | 6.7 | 6.7 | 6.0 |
| CC(ano) | -0.076 | -0.126 | -0.005 |
| |DANO|/σ(DANO) | 0.8 | 0.7 | 0.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 2.0 M ammonium sulfate |
