7SKS
Crystal structure of measles virus matrix protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 85.954, 85.954, 402.506 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.541 |
| R-factor | 0.2319 |
| Rwork | 0.231 |
| R-free | 0.25740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bk6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.940 |
| Data reduction software | XDS (Feb 5, 2021) |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.998 | 19.998 | 2.585 |
| High resolution limit [Å] | 2.541 | 6.811 | 2.541 |
| Rmerge | 0.143 | 0.056 | 6.616 |
| Rmeas | 0.144 | 0.056 | 6.706 |
| Rpim | 0.015 | 0.006 | 1.079 |
| Total number of observations | 2554941 | 138489 | 55551 |
| Number of reflections | 30225 | 1722 | 1461 |
| <I/σ(I)> | 29.31 | 85.57 | 0.69 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 84.53 | 80.42 | 38.02 |
| CC(1/2) | 1.000 | 1.000 | 0.442 |
| Anomalous redundancy | 46.6 | 51.2 | 20.2 |
| CC(ano) | -0.033 | 0.004 | 0.002 |
| |DANO|/σ(DANO) | 0.7 | 0.7 | 0.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 19% (v/v) isopropanol, 19% (w/v) PEG 4000, 5% (v/v) glycerol, 0.095 M sodium citrate pH 5.6 |
