7SKJ

Crystal structure of chloroplast triosephosphate isomerase from Cuscuta australis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2021-08-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9464
Spacegroup name	P 21 21 21
Unit cell lengths	74.949, 82.118, 154.246
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.970 - 1.900
R-factor	0.1866
Rwork	0.185
R-free	0.23520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ohq
RMSD bond length	0.008
RMSD bond angle	0.859
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.970	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	75939	4271
<I/σ(I)>	16.3
Completeness [%]	99.8
Redundancy	13.6
CC(1/2)	0.999	0.928

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M Ammonium acetate, 0.1 M HEPES pH 7.5, 25% w/v PEG 3350