7SKG

Myocilin OLF mutant V329M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-07-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	49.479, 50.730, 50.508
Unit cell angles	90.00, 96.22, 90.00

Refinement procedure

Resolution	35.690 - 1.330
R-factor	0.1673
Rwork	0.167
R-free	0.18330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pke
RMSD bond length	0.005
RMSD bond angle	0.879
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.690	1.378
High resolution limit [Å]	1.330	1.330
Rmerge	0.087	0.501
Rmeas	0.095	0.554
Rpim	0.039	0.234
Number of reflections	56446	5575
<I/σ(I)>	23.02	5.58
Completeness [%]	98.7	98.03
Redundancy	5.8	5.4
CC(1/2)	0.998	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	289	10% PEG 8000, 0.05M magnesium chloride