7SKF

Myocilin OLF mutant A445V

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-06-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	49.349, 50.653, 50.629
Unit cell angles	90.00, 96.49, 90.00

Refinement procedure

Resolution	33.280 - 1.282
R-factor	0.1621
Rwork	0.162
R-free	0.16630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pke
RMSD bond length	0.004
RMSD bond angle	0.863
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.280	1.328
High resolution limit [Å]	1.282	1.282
Rmerge	0.047	0.157
Rmeas	0.052	0.172
Rpim	0.020	0.068
Number of reflections	62664	6143
<I/σ(I)>	25.72	8.92
Completeness [%]	98.4	96.74
Redundancy	6.3	6.1
CC(1/2)	0.999	0.983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	289	10% PEG 8000, 0.05M magnesium chloride