7SKE

Myocilin OLF mutant R296H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-06-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	49.243, 50.721, 50.364
Unit cell angles	90.00, 97.67, 90.00

Refinement procedure

Resolution	35.580 - 1.240
R-factor	0.174
Rwork	0.173
R-free	0.19250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pke
RMSD bond length	0.004
RMSD bond angle	0.860
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.580	1.284
High resolution limit [Å]	1.240	1.240
Rmerge	0.073	0.479
Rmeas	0.080	0.546
Rpim	0.033	0.255
Number of reflections	67993	5562
<I/σ(I)>	12.59	1.78
Completeness [%]	97.5	80.39
Redundancy	5.7	3.9
CC(1/2)	0.998	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	289	8% PEG 8000, 0.05M magnesium chloride