7SJW

Myocilin OLF mutant L303I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-04-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	49.331, 50.891, 50.496
Unit cell angles	90.00, 96.40, 90.00

Refinement procedure

Resolution	35.310 - 1.380
R-factor	0.1623
Rwork	0.162
R-free	0.17930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pke
RMSD bond length	0.005
RMSD bond angle	0.855
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.310	1.429
High resolution limit [Å]	1.380	1.380
Rmerge	0.120	0.471
Rmeas	0.131	0.518
Rpim	0.052	0.211
Number of reflections	50211	4765
<I/σ(I)>	25.3	7.03
Completeness [%]	97.8	93.83
Redundancy	5.9	5.5
CC(1/2)	0.994	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	10% PEG 8000, 0.05M magnesium chloride