7SJU

Myocilin OLF mutant T293K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-04-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	49.376, 50.602, 50.432
Unit cell angles	90.00, 96.75, 90.00

Refinement procedure

Resolution	35.600 - 1.391
R-factor	0.1614
Rwork	0.161
R-free	0.17950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pke
RMSD bond length	0.005
RMSD bond angle	0.877
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.600	1.440
High resolution limit [Å]	1.391	1.391
Rmerge	0.068	0.186
Rmeas	0.074	0.202
Rpim	0.029	0.077
Number of reflections	48219	4536
<I/σ(I)>	26.38	10.21
Completeness [%]	97.0	92.08
Redundancy	6.5	6.7
CC(1/2)	0.997	0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	8% PEG 8000, 0.1M magnesium chloride