7SJ5

Bacteriophage lambda major capsid protein mutant - W308A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-09-30
Detector	CUSTOM-MADE
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	170.153, 95.303, 113.121
Unit cell angles	90.00, 127.92, 90.00

Refinement procedure

Resolution	29.750 - 2.695
Rwork	0.207
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bqw
RMSD bond length	0.002
RMSD bond angle	0.457
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHENIX
Refinement software	PHENIX (dev-3724)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.750	2.791
High resolution limit [Å]	2.695	2.695
Rmerge	0.056
Rmeas	0.065
Rpim	0.034
Number of reflections	39376	3819
<I/σ(I)>	18.27
Completeness [%]	99.5
Redundancy	3.7
CC(1/2)	0.999	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	291.15	0.1 M bis-tris, 0.1 M ammonium sulfate, 5% v/v glycerol, 30% v/v PEG-3350