7SIZ

C-type inactivation in a voltage gated K+ channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	90
Detector technology	PIXEL
Collection date	2019-06-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	P 4 21 2
Unit cell lengths	130.732, 130.732, 278.858
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.750 - 3.100
R-factor	0.2371
Rwork	0.236
R-free	0.26480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r9r
RMSD bond length	0.006
RMSD bond angle	0.600
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.750	3.220
High resolution limit [Å]	3.100	3.100
Rpim	0.070	0.960
Number of reflections	46801	4607
<I/σ(I)>	10.1	1
Completeness [%]	100.0
Redundancy	13.1
CC(1/2)	0.990	0.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	293	50 mM Tris, pH 8.6, 28-30% PEG400, 8 mM CHAPS