7SIQ
Crystal structure of a peptide chain release factor 3 (prfC) from Stenotrophomonas maltophilia bound to GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 287 |
Detector technology | CCD |
Collection date | 2020-06-25 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9787 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 96.540, 96.540, 170.070 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.270 - 2.950 |
R-factor | 0.2263 |
Rwork | 0.224 |
R-free | 0.27860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tr5 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (dev-4274) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.270 | 48.270 | 3.030 |
High resolution limit [Å] | 2.950 | 13.190 | 2.950 |
Rmerge | 0.068 | 0.027 | 0.732 |
Rmeas | 0.071 | 0.029 | 0.767 |
Total number of observations | 216402 | ||
Number of reflections | 19936 | 266 | 1454 |
<I/σ(I)> | 22.82 | 54.19 | 4 |
Completeness [%] | 99.8 | 95 | 99.7 |
Redundancy | 10.855 | 8.132 | 11.162 |
CC(1/2) | 0.999 | 0.999 | 0.991 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 287 | StmaA.18013.a.B1.PW38802 at 20.5 mg/mL with 3 mM MgCl2 and 3 mM GDP against Morpheus screen condition D5 containing 10% PEG 20,000, 20% PEG 550 MME, 0.02 mM each 1,6-hexanediol, 1-butanol, 1,2-propanediol, 2-propanol, 1,4-butanediol, 1,3-propanediol, 0.1 M MOPES/Hepes pH 7.5, direct cryo, unique puck ID tja6-9, crystal tracking ID 315959d5 |