7SIJ
Myocilin OLF mutant E352K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2021-03-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.336, 50.734, 50.490 |
Unit cell angles | 90.00, 96.60, 90.00 |
Refinement procedure
Resolution | 37.260 - 1.540 |
R-factor | 0.1563 |
Rwork | 0.155 |
R-free | 0.17780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6pke |
RMSD bond length | 0.005 |
RMSD bond angle | 0.867 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.260 | 1.595 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.051 | 0.153 |
Rmeas | 0.055 | 0.167 |
Rpim | 0.022 | 0.066 |
Number of reflections | 36260 | 3465 |
<I/σ(I)> | 22.48 | 8.21 |
Completeness [%] | 98.6 | 95.58 |
Redundancy | 6.4 | 6.3 |
CC(1/2) | 0.999 | 0.985 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 289 | 10% PEG 8000, 0.25M magnesium chloride |