7SGO
Crystal structure of periplasmic domain of Helicobacter pylori FliL (residues 81 to 183) (crystal form B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-10-02 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.350, 42.548, 140.585 |
| Unit cell angles | 90.00, 93.29, 90.00 |
Refinement procedure
| Resolution | 46.784 - 2.695 |
| R-factor | 0.1861 |
| Rwork | 0.184 |
| R-free | 0.23130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.887 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.784 | 2.830 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.064 | 0.336 |
| Number of reflections | 25074 | 3405 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 93.0 | |
| Redundancy | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | Ammonium di-hydrogen phosphate, trisodium citrate |
