7SG9
[mC-mC] DNA mismatch in a self-assembling rhombohedral lattice at pH 5.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-18 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 1.00743 |
Spacegroup name | H 3 |
Unit cell lengths | 106.006, 106.006, 93.781 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.800 - 3.810 |
R-factor | 0.2346 |
Rwork | 0.231 |
R-free | 0.31360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5w6w |
RMSD bond length | 0.006 |
RMSD bond angle | 0.946 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 65.603 | 65.603 | 4.096 |
High resolution limit [Å] | 3.809 | 10.307 | 3.809 |
Number of reflections | 2745 | 195 | 196 |
<I/σ(I)> | 12.2 | 42.6 | 0.8 |
Completeness [%] | 89.1 | 100 | 59.9 |
Redundancy | 10.9 | 10.6 | |
CC(1/2) | 0.999 | 0.999 | 0.368 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | MOPS, Magnesium sulfate |