7SG6

Structure of PfCSP peptide 21 with antibody CIS43_Var10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	298
Detector technology	PIXEL
Collection date	2021-09-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	93.790, 61.570, 74.984
Unit cell angles	90.00, 105.93, 90.00

Refinement procedure

Resolution	44.960 - 1.550
R-factor	0.1857
Rwork	0.184
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b5m
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.960	1.600
High resolution limit [Å]	1.550	1.550
Rmerge	0.132	0.846
Number of reflections	58909	5896
<I/σ(I)>	5.6
Completeness [%]	98.6
Redundancy	3.5
CC(1/2)	0.985	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	15% ISOPROPANOL, 0.2M AMMONIUM CITRATE pH 3.5, 25% PEG 3,350