7SG5

Structure of PfCSP peptide 21 with antibody CIS43_Var2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	298
Detector technology	PIXEL
Collection date	2021-09-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	94.770, 61.137, 75.313
Unit cell angles	90.00, 106.56, 90.00

Refinement procedure

Resolution	50.720 - 1.400
R-factor	0.1867
Rwork	0.185
R-free	0.21760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b5m
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.720	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.044	0.349
Number of reflections	61516	4587
<I/σ(I)>	14.71
Completeness [%]	75.5
Redundancy	2.1
CC(1/2)	0.996	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M ammonium sulfate, 28% w/v polyethylene glycol 4,000