7SFY

Crystal structure of human Mis18ab_cc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	193.15
Detector technology	CCD
Collection date	2017-08-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	C 2 2 21
Unit cell lengths	77.501, 101.815, 88.563
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.280 - 2.500
R-factor	0.2494
Rwork	0.248
R-free	0.27960
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.069
Data reduction software	xia2
Data scaling software	xia2
Phasing software	AutoSol
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.078
Number of reflections	12467	600
<I/σ(I)>	9.2
Completeness [%]	99.0
Redundancy	5.2
CC(1/2)	0.995	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	Magnesium acetate, PEG 3350