7SFX
10A1 Fab in complex with CD99 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.410, 131.550, 119.811 |
| Unit cell angles | 90.00, 90.12, 90.00 |
Refinement procedure
| Resolution | 46.380 - 3.100 |
| R-factor | 0.2318 |
| Rwork | 0.226 |
| R-free | 0.24770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xza |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.055 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmeas | 0.157 | 0.456 |
| Rpim | 0.069 | 0.198 |
| Number of reflections | 36430 | 1834 |
| <I/σ(I)> | 10.97 | 4.35 |
| Completeness [%] | 98.9 | 99.3 |
| Redundancy | 5.1 | 5.2 |
| CC(1/2) | 0.991 | 0.868 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.2 M lithium sulfate monohydrate, 0.1 M HEPES, pH 7.3, 25% PEG3350, and 0.1 M L-Proline |
