7SFM

Mycobacterium tuberculosis Hip1 crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-09
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9436
Spacegroup name	P 31 2 1
Unit cell lengths	104.891, 104.891, 127.802
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.464 - 2.149
R-factor	0.1759
Rwork	0.174
R-free	0.20490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ugq
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.464	2.230
High resolution limit [Å]	2.149	2.149
Rmeas	0.000	0.000
Number of reflections	44555	4379
<I/σ(I)>	17
Completeness [%]	99.0
Redundancy	1.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	30% PEG 3350, 0.1M sodium acetate pH 5.6, 0.2 M ammonium sulfate and 10% glycerol