7SF5
M. tb EgtD in complex with HD3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-08-31 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.146, 70.277, 143.883 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.270 - 2.520 |
R-factor | 0.2033 |
Rwork | 0.198 |
R-free | 0.26760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4uy5 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.270 | 2.610 |
High resolution limit [Å] | 2.520 | 2.520 |
Number of reflections | 24381 | 2384 |
<I/σ(I)> | 11 | |
Completeness [%] | 99.9 | |
Redundancy | 1 | |
CC(1/2) | 0.980 | 0.550 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.2 M potassium phosphate dibasic and 20 % w/v polyethylene glycol 3,350 |