7SF2
Crystal Structure of Beta-Galactosidase from Bacteroides cellulosilyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-10 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 335.847, 349.396, 352.108 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.100 - 2.750 |
| R-factor | 0.1831 |
| Rwork | 0.182 |
| R-free | 0.20940 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.494 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.110 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.149 | 0.658 |
| Number of reflections | 255513 | 12805 |
| <I/σ(I)> | 8.3 | 2.4 |
| Completeness [%] | 96.5 | |
| Redundancy | 4.8 | |
| CC(1/2) | 0.987 | 0.798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1 M Tris-HCl, pH 8.5, 2.4 M ammonium phosphate dibasic |
