7SF2
Crystal Structure of Beta-Galactosidase from Bacteroides cellulosilyticus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-10 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 335.847, 349.396, 352.108 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.100 - 2.750 |
R-factor | 0.1831 |
Rwork | 0.182 |
R-free | 0.20940 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.494 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.110 | 2.800 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.149 | 0.658 |
Number of reflections | 255513 | 12805 |
<I/σ(I)> | 8.3 | 2.4 |
Completeness [%] | 96.5 | |
Redundancy | 4.8 | |
CC(1/2) | 0.987 | 0.798 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1 M Tris-HCl, pH 8.5, 2.4 M ammonium phosphate dibasic |