7SF0

Crystal structure of Vaccinia Virus decapping enzyme D9 in complex with trinucleotide substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.115869
Spacegroup name	C 2 2 21
Unit cell lengths	52.740, 139.990, 76.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.995 - 1.950
R-factor	0.205288343236
Rwork	0.201
R-free	0.24709
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7sez
RMSD bond length	0.005
RMSD bond angle	0.919
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.083	1.142
Number of reflections	20891	2021
<I/σ(I)>	20.86
Completeness [%]	99.8
Redundancy	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium sulfate, PEG3350, Bis Tris propane pH 7.5, magnesium chloride