7SEZ

Crystal structure of Vaccinia Virus decapping enzyme D9 in complex with m7GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.115869
Spacegroup name	C 2 2 21
Unit cell lengths	52.550, 140.100, 75.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.502 - 1.700
R-factor	0.195459436044
Rwork	0.193
R-free	0.22450
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.218
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.502	1.761
High resolution limit [Å]	1.700	1.700
Rmerge	0.053
Rmeas	0.055
Number of reflections	31249	3061
<I/σ(I)>	26.5
Completeness [%]	99.8
Redundancy	12.8
CC(1/2)		0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium citrate, PEG 3350, Bis Tris propane pH 7.5, MgCl2