7SEX

M. tb EgtD in complex with TGX221

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-31
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.980
Spacegroup name	P 41 21 2
Unit cell lengths	69.019, 69.019, 140.922
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.310 - 2.200
R-factor	0.1856
Rwork	0.181
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uy5
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.310	2.279
High resolution limit [Å]	2.200	2.201
Rmerge	0.080
Number of reflections	17958	1712
<I/σ(I)>	10
Completeness [%]	99.8
Redundancy	1
CC(1/2)	0.950	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.2 M potassium phosphate dibasic and 20 % w/v polyethylene glycol 3,350