7SEU

Crystal structure of E coli contaminant protein YncE co-purified with a plant protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-13
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	53.172, 147.270, 96.899
Unit cell angles	90.00, 104.40, 90.00

Refinement procedure

Resolution	48.610 - 2.500
R-factor	0.237
Rwork	0.236
R-free	0.25590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold AF-P76116-F1
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.560
High resolution limit [Å]	2.500	2.500
Number of reflections	87600	3604
<I/σ(I)>	7.3
Completeness [%]	95.9
Redundancy	2.8
CC(1/2)	0.981	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2 M Li2SO4, 0.1 M MES, pH 6.0, and 20% PEG 4000