7SER
PRMT5/MEP50 with compound 30 bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 103.891, 138.305, 179.191 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.930 - 2.140 |
R-factor | 0.2079 |
Rwork | 0.206 |
R-free | 0.25260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7s0u |
RMSD bond length | 0.007 |
RMSD bond angle | 0.828 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.140 | 2.140 |
Number of reflections | 61203 | 1684 |
<I/σ(I)> | 16.1 | |
Completeness [%] | 85.9 | |
Redundancy | 2.8 | |
CC(1/2) | 0.990 | 0.910 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 100 mM Sodium Citrate pH 5.4, 12% PEG 3350, 4% Tascimate pH 5 |