7SEB

Crystal structure of human Fibrillarin in complex with compound 2 from single soak

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-28
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	C 2 2 21
Unit cell lengths	69.210, 138.905, 66.483
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.030 - 1.810
R-factor	0.1961
Rwork	0.194
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ipx
RMSD bond length	0.011
RMSD bond angle	1.091
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.030	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.078	1.658
Rmeas	0.081	1.733
Rpim	0.023	0.495
Number of reflections	29246	1359
<I/σ(I)>	18	1.2
Completeness [%]	97.5
Redundancy	13.1
CC(1/2)	0.999	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.75-3.3 M Sodium Formate