7SE6
Crystal structure of human Fibrillarin in ligand-free state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-03-10 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9537 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 69.145, 138.186, 66.551 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.330 - 1.990 |
R-factor | 0.2283 |
Rwork | 0.226 |
R-free | 0.25950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ipx |
RMSD bond length | 0.013 |
RMSD bond angle | 1.141 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.930 | 2.040 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.053 | 1.087 |
Rmeas | 0.057 | 1.172 |
Rpim | 0.021 | 0.433 |
Number of reflections | 22206 | 1623 |
<I/σ(I)> | 23.7 | 1.7 |
Completeness [%] | 99.9 | |
Redundancy | 7.3 | |
CC(1/2) | 1.000 | 0.821 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.75-3.3 M Sodium Formate |