7SD2

Murine Fab that recognizes Hev b 8 (profilin for Hevea brasiliensis)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-21
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	I 1 2 1
Unit cell lengths	91.000, 103.845, 180.204
Unit cell angles	90.00, 94.26, 90.00

Refinement procedure

Resolution	44.960 - 3.750
R-factor	0.2484
Rwork	0.246
R-free	0.28410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1baf
RMSD bond length	0.002
RMSD bond angle	0.557
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.920	4.190
High resolution limit [Å]	3.750	3.750
Rmerge	0.129
Rpim	0.084
Number of reflections	17207	4862
<I/σ(I)>	8.1
Completeness [%]	99.1	99.8
Redundancy	3.3
CC(1/2)	0.991	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.9	291	0.2 M magnesium acetate tetrahydrate, 20% w/v PEG3350