7SCM
Crystal Structure of Ancestral Amniote Cadherin-23 EC1-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 73.109, 73.109, 381.934 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.930 - 2.272 |
| Rwork | 0.190 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2whv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.534 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmeas | 0.492 | 4.487 |
| Number of reflections | 29083 | 1389 |
| <I/σ(I)> | 16 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 26.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1 M Tris pH 7.5 3 M Sodium formate Ancestral Amniote Protocadherin-15 EC1-2 |
