7SBM
Human glutaminase C (Y466W) with L-Gln, open conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-04-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 98.842, 139.100, 177.435 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.290 - 2.800 |
R-factor | 0.2018 |
Rwork | 0.200 |
R-free | 0.24280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d3o |
RMSD bond length | 0.009 |
RMSD bond angle | 1.301 |
Data reduction software | HKL-2000 (721.2) |
Data scaling software | HKL-2000 (721.2) |
Phasing software | PHENIX (1.15.2_3472) |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.300 | 2.850 |
High resolution limit [Å] | 2.800 | 2.810 |
Rpim | 0.061 | 0.251 |
Number of reflections | 60927 | 5953 |
<I/σ(I)> | 4.2 | |
Completeness [%] | 99.4 | 98.52 |
Redundancy | 6.3 | 6.3 |
CC(1/2) | 0.993 | 0.948 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 11.5% PEG 6000, 0.95 M LiCl, and 0.1 M Tris pH 8.5 |