7SBJ
Crystal Structure of Ribulose-phosphate 3-epimerase from Stenotrophomonas maltophilia K279a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-09-16 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 83.890, 136.480, 148.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.740 - 1.850 |
R-factor | 0.1486 |
Rwork | 0.148 |
R-free | 0.17880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5umf as per Morda |
RMSD bond length | 0.008 |
RMSD bond angle | 0.880 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MoRDa |
Refinement software | PHENIX (1.19 dev 4359) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.900 |
High resolution limit [Å] | 1.850 | 8.270 | 1.850 |
Rmerge | 0.095 | 0.030 | 0.496 |
Rmeas | 0.102 | 0.033 | 0.533 |
Number of reflections | 72940 | 907 | 5361 |
<I/σ(I)> | 17.49 | 47.98 | 4.37 |
Completeness [%] | 100.0 | 99 | 99.9 |
Redundancy | 7.446 | 6.547 | 7.456 |
CC(1/2) | 0.998 | 0.998 | 0.927 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 287 | Anatrace/Calibre MCSG1 screen, condition e7: 200mM ammonium iodide, 20% (w/V) PEG 3350: StmaA.18161.a.B1.PS38644 at 18.6mg/ml, tray: 321485e7, puck: pxu9-1: cryo: 20% EG |