7SBI
Tandem diubiquitin-like domain from chicken 2'-5'-oligoadenylate synthetase-like protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-20 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 28.921, 100.922, 55.748 |
Unit cell angles | 90.00, 104.96, 90.00 |
Refinement procedure
Resolution | 27.940 - 2.230 |
R-factor | 0.2307 |
Rwork | 0.229 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Homology Model |
RMSD bond length | 0.002 |
RMSD bond angle | 0.549 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (phenix-1.18.2-3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.530 | 2.290 |
High resolution limit [Å] | 2.230 | 2.230 |
Rpim | 0.075 | 1.100 |
Number of reflections | 13956 | 1176 |
<I/σ(I)> | 8.3 | 1.3 |
Completeness [%] | 93.8 | 79.6 |
Redundancy | 2.9 | 2.3 |
CC(1/2) | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | tri-ammonium citrate, PEG 3350, galactose |