7SBG
Murine Fab/IgE in complex with profilin from Hevea brasieliensis (Hev b 8)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-04 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.325, 71.450, 132.518 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.570 - 3.340 |
R-factor | 0.2525 |
Rwork | 0.245 |
R-free | 0.29780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7sbd |
RMSD bond length | 0.003 |
RMSD bond angle | 0.625 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.220 | 3.350 |
High resolution limit [Å] | 3.230 | 3.230 |
Rmerge | 0.432 | |
Number of reflections | 7675 | 749 |
<I/σ(I)> | 16.65 | |
Completeness [%] | 98.8 | 91.89 |
Redundancy | 1.8 | 1.8 |
CC(1/2) | 0.993 | 0.670 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 291 | 0.2 M lithium acetate, 20% PEG3350 |