7SBG
Murine Fab/IgE in complex with profilin from Hevea brasieliensis (Hev b 8)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-04 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.325, 71.450, 132.518 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.570 - 3.340 |
| R-factor | 0.2525 |
| Rwork | 0.245 |
| R-free | 0.29780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7sbd |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.625 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.220 | 3.350 |
| High resolution limit [Å] | 3.230 | 3.230 |
| Rmerge | 0.432 | |
| Number of reflections | 7675 | 749 |
| <I/σ(I)> | 16.65 | |
| Completeness [%] | 98.8 | 91.89 |
| Redundancy | 1.8 | 1.8 |
| CC(1/2) | 0.993 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 291 | 0.2 M lithium acetate, 20% PEG3350 |
